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ROLE OF SUSPENDED SEDIMENT IN FACILITATING PATHOGEN TRANSPORT
IN INNER BLUEGRASS KARST AQUIFERS

Principal Investigator:
Alan Fryar, Geological Sciences

Co-Principal Investigators:
Gail Brion, Civil Engineering
Mark S. Coyne, Department of Agronomy
Joe Taraba, Biosystems and Agricultural Engineering
Alex Fogle, Kentucky Geological Survey
Tom Reed, Geological Sciences

Keywords:
karst environment, suspended sediment, pathogens, coliform

Brief Project Summary

Almost 40% of Kentucky is underlain by limestone, which is prone to dissolution (karstification). Because karstification results in the development of preferential flow paths through sinkholes and subsurface conduits, karst aquifers are especially vulnerable to nonpoint-source pollution from urban and agricultural runoff. Suspended sediment, which can be carried into the subsurface or remobilized within karst conduits following storms, can adsorb pathogens and other pollutants. However, there have been few studies of the association between pathogens and sediment in karst aquifers in Kentucky, and none in the Inner Bluegrass region.

The objectives of this study were to characterize the relationship between suspended sediment and pathogen indicators at two springs draining karst basins with contrasting land uses (urban and agricultural) in Woodford County, Kentucky. Blue Hole Spring is located in Versailles; spring SP-2 is at the University of Kentucky (UK) Animal Research Center (ARC). At each site, specific conductance (SC), pH, and temperature (T) were manually measured weekly from fall 2002 to spring 2004. Concentrations of fecal coliform bacteria (FC), total coliforms (TC), atypical coliforms (AC), male-specific coliphage (MSP, an indicator of viruses in waste water), and major ions were measured biweekly. Discharge at SP-2, stream stage at Blue Hole (which has been correlated to discharge), SC, pH, T, and turbidity were monitored continuously by sensors linked to digital data loggers. Bed sediments at SP-2 and the sinkhole feeding it were analyzed for mineralogy, petrology, particle size, total organic carbon (TOC), and total inorganic carbon (TIC). During two storms, SC, T, FC, TC, AC, MSP, and total suspended solids (TSS) were measured at both springs, and suspended sediment samples were collected at SP-2 for the same analyses previously performed on bed sediments.

 


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